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Journal of Histochemistry & Cytochemistry
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Journal of Histochemistry & Cytochemistry
Article . 1988 . Peer-reviewed
License: https://journals.sagepub.com/page/policies/text-and-data-mining-license
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Journal of Histochemistry & Cytochemistry
Article . 1988
Data sources: Europe PubMed Central
Journal of Histochemistry & Cytochemistry
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Label-fracture of cell surfaces by replica staining.

descriptionPublicationkeyboard_double_arrow_right Article 01 Nov 1988Publisher:SAGE PublicationsJournal:Journal of Histochemistry & Cytochemistry, volume 36, pages 1,413-1,418 (issn: 0022-1554, eissn: 1551-5044, Copyright policy )
Authors: C.A. Forsman; Pedro Pinto da Silva;

doi: 10.1177/36.11.2459187

pmid: 2459187

Label-fracture of cell surfaces by replica staining.

Abstract

We introduce replica-staining label-fracture, a method for the cytochemical mapping of membrane surfaces. This method is a corollary of the rationale of label-fracture (Pinto da Silva and Kan, 1984: J Cell Biol 99:1156). After freeze-fracture the exoplasmic halves of the membrane remain attached to the replica. We show that cytochemical labeling of cell surfaces can be performed by direct post-fracture staining of freeze-fracture replicas. This new variant of label-fracture leads to miniaturization of labeling procedures and allows standardization of labeling conditions and simultaneous processing of different specimens.

Keywords

Staining and Labeling, Histocytochemistry, Wheat Germ Agglutinins, Cell Membrane, Erythrocyte Membrane, Immunohistochemistry, Concanavalin A, Freeze Fracturing, Humans, Colloids, Gold, Lymphocytes

  • BIP!
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    citations
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 28
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    		 Average
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 Top 10%
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    		 Top 10%
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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
28
Average
Top 10%
Top 10%
bronze