The mechanical and antibacterial properties of acrylic rubber/poly(methyl methacrylate) (AR/PMMA) blend at 10 to 50 wt% of AR content with non-treated and treated titanium dioxide (TiO2) and 2-Hydroxypropyl-3-piperazinyl-quinoline carboxylic acid methacrylate (HPQM) by N-2(aminoethyl)-3-aminopropyl trimethoxysilane were studied. The antibacterial property against Escherichia coli was evaluated. The results found that the mechanical properties of ARt-TiO2/PMMA and ARt-HPQM/PMMA blend were higher than that of the ARTiO2/PMMA and ARHPQM/PMMA blend. For antibacterial property, the ARHPQM/PMMA and ARt-HPQM/PMMA blend could act as the antibacterial material, while the ARTiO2/PMMA blend did not show. However, the ARt-TiO2/PMMA blend could inhibit bacterial cell growth with 10 to 30 wt% of AR content. The recommended compositions of ARt-TiO2/PMMA blend, which improved both mechanical and antibacterial properties, were 10 to 30 wt% of AR and were 10 to 50 wt% of AR for ARt-HPQM/PMMA. Moreover, the UV radiation increased the antibacterial properties by the destruction of the interaction in treated TiO2 and HPQM and improved the antibacterial performance of ARt-TiO2/PMMA and ARt-HPQM/PMMA blend.