Hepatitis A is a benign infection, which in the developing world affects mainly children; the majority of adults are immune by the age of 30. In the last decade or so, a shift in the prevalence pattern of hepatitis A virus (HAV) infection from a low- to a high-age group has appeared in the developed countries. This shift has been attributed to an improvement in the socio-economic and hygienic conditions. In the present study, 849 Syrians of all age groups and both sexes were tested by enzyme-linked immunosorbent assay technique for the seroprevalence of hepatitis A IgG antibody (anti-HAV IgG). It was observed that anti-HAV IgG was present in 89% Syrian population; with 50% in the 1–5 year age group and 95% in the 11–15 year age group. These results demonstrate that HAV infection in Syria is mostly acquired during childhood. As yet there is no serological evidence of a shift in HAV infection from a younger to a higher age group.