The purpose of this study was the effect of icing on the muscle activity of Hamstring muscles during Nordic Hamstring Exercise after applying the icing. Twenty-eight healthy men participated in this study. Subjects were assigned to experiment and control groups. Nordic Hamstring Exercise was practiced to the experiment and the control groups without applying the icing. The control group rested for 20 minutes with no intervention, while the experiment group was applied 20 minutes of icing. Then Nordic Hamstring Exercise was performed again to compare the differences in muscle activity between the two groups. No significant differences in muscle activity were observed in the control group. However, In the experimental group, significant differences in muscle activity were found pre and post icing. There were also significant differences between the two groups. In conclusion, icing is considered to have a positive effect on promoting hamstring muscle activity.