
doi: 10.1159/000148850
pmid: 4775752
The total carbohydrate content of purified hepatitis B antigen (HB Ag) ranged between 3.6 and 6.5% as determined by the phenol-sulfuric acid method. Disc-sodium dodecyl sulfate PAGE of purified HB Ag followed by periodate-Schiff staining revealed one major and two minor glycoproteins with molecular weights of 22,000,27,000 and 32,000 daltons, respectively. Both ad and ay subtypes of HB Ag contained these glycoproteins.
Carbohydrates, Sulfuric Acids, Electrophoresis, Disc, Hepatitis B Antigens, Molecular Weight, Viral Proteins, Phenols, Centrifugation, Density Gradient, Methods, Serotyping, Peptides, Glycoproteins
Carbohydrates, Sulfuric Acids, Electrophoresis, Disc, Hepatitis B Antigens, Molecular Weight, Viral Proteins, Phenols, Centrifugation, Density Gradient, Methods, Serotyping, Peptides, Glycoproteins
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