Growth and physiological variability among clones ofDalbergia sissoogrowing in a CSO revealed maximum height and GBH in Gonda clones (C196 and C198) and minimum growth attributes in Rajasthan clones. All biochemical constituents except sugar were also maximum in Gonda clones. Maximum chl. a, total chl., and chlorofluorescence (CF) were recorded in C235 and C123 while chl. b was maximum in C198. Among tested clones, sugar content was maximum in C60 (Chhachhrauli) while C198 (Gonda) revealed maximum protein content. Heritability estimates of 8 characters at 99% revealed strong genetic control of total chls., sugars, proteins, and chl. b; however, maximum genetic gains of 117% and 80% were recorded for sugar and protein content, respectively. Correlation matrix revealed a positive correlation between height and GBH and CF. Among biochemical constituents, chl. a, and chl. b, chl. b, and total chl. were correlated significantly at 0.1% level. Regarding contribution of different parameters to variability, height and GBH were the greatest contributors. Clustering of clones on the basis of all three parameters separated clones in one major and six minor clusters. Average distance from centroid was found to be 22.61 whereas the maximum distance from centroid was 50.75.