The aim of the present paper was to observe the effects of needling ST40 and PC6 on IL‐17 of ApoE−/− mice with fatty liver. Forty male ApoE−/− mice were randomized into Needling‐Acupoint Group, Simvastatin Intragastric Administration Group, Needling Nonacupoint Group, and Model Group. Each was fed with high fat diet for 8 weeks since 16 weeks of age; after 8 weeks of intervention, mice were sacrificed and tested for various examinations. Result showed that the body weight, TC, and serum IL‐17 in Needling‐Acupoint Group decreased. Compared with Model Group, the immunohistochemical expressions of IL‐17 in liver tissue were significantly decreased among the other three groups. In conclusion, acupuncture was able to lower the expression of IL‐17 level both in serum and liver tissue in ApoE−/− mice, which is helpful to reduce the inflammation and defers the progress from fatty liver to cirrhosis.