In order to generate color test image consistent with human perception in aspect of saturation, lightness, and hue of image, we propose a saturation test image design method based on CIEDE2000 color difference formula. This method exploits the subjective saturation parameterC′ of CIEDE2000 to get a series of test images with different saturation but same lightness and hue. It is found experimentally that the vision perception has linear relationship with the saturation parameterC′. This kind of saturation test image has various applications, such as in the checking of color masking effect in visual experiments and the testing of the visual effects of image similarity component.