DNA-Dependent RNA Polymerase from Tetrahymena pyriformis

descriptionPublicationkeyboard_double_arrow_right Article 01 Feb 1972 English Publisher:Canadian Science PublishingJournal:Canadian Journal of Biochemistry, volume 50, pages 154-157 (issn: 0008-4018,

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Authors: S, Kurtz; R E, Pearlman;

doi: 10.1139/o72-020

pmid: 4622563

DNA-Dependent RNA Polymerase from Tetrahymena pyriformis

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Abstract

DNA-dependent RNA polymerase has been isolated from sonicated nuclei of the ciliate protozoan Tetrahymena pyriformis. The enzyme has been further purified by column chromatography on DEAE-Sephadex and is approximately 300-fold purified relative to total cellular protein.

Keywords

Cell Nucleus, Manganese, Deoxyribonucleases, Tetrahymena pyriformis, RNA Nucleotidyltransferases, DNA, Mycotoxins, Chromatography, Ion Exchange, Nucleic Acid Denaturation, Vibration, Enzyme Activation, Ammonium Sulfate, Hydroxides, Potassium, Animals, Chemical Precipitation, Magnesium, Rifampin

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