Observations on Ampelomyces quisqualis disclosed that the pycnidium may originate either from one cell of a single pycnidiospore, or from one hyphal cell. In the first case the pycnidiospore becomes two celled and swollen and a profuse germination of one of the two swollen spore cells can be observed. Later, the short hyphae branches, interweave, and anastomose to form a compact network around the mother spore, the pycnidium primordium. Similarly, we observed profuse branching in a single hyphal cell. The newly formed branches interweave and anastomose to form a compact network which gives rise to the pycnidium primordium. Hyphal rings were also observed throughout this study, but no pycnidia arose from these structures. During the vegetative growth of the fungus, hyphal anastomosis seems to be a frequent pattern. It seems that the pycnidial ontogeny of A. quisqualis does not conform to any known developmental type.