descriptionPublicationkeyboard_double_arrow_right Article 01 Aug 1972 English Publisher:Canadian Science PublishingJournal:Canadian Journal of Microbiology, volume 18, pages 1,241-1,245 (issn: 0008-4166, eissn: 1480-3275,

Authors: Miyoshi Ikawa; Ashima Chakravarti; Richard F. Taylor;

doi: 10.1139/m72-192

pmid: 5052892

Occurrence of choline in Lactobacillus plantarum

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Abstract

When Lactobacillus plantarum 8014 was grown on a Trypticase Soy Broth (TSB) type of medium, choline accumulated within the cells, whereas when the same organism was grown on a semisynthetic medium, no choline accumulation was observed. Only the addition of choline O-sulfate resulted in the accumulation of choline in cells grown on semisynthetic medium to the extent seen in cells grown on TSB medium. The choline of TSB-grown cells is shown to be non-lipoidal and to occur in the cells as free choline, choline O-sulfate, and choline O-phosphate. No choline was detected in the cell wall of the choline-containing cells.

Keywords

Lactobacillus, Solubility, Cell Wall, Chromatography, Paper, Chromatography, Thin Layer, Choline, Culture Media

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