Naphthylamidases of Sarcina lutea
Copyright policy )Naphthylamidases of Sarcina lutea
The naphthylamidase isozyme complement of Sarcina lutea was studied. Gel filtration yielded two fractions, Sephadex I and Sephadex II. Sephadex I contained one enzyme generally resembling leucineaminopeptidase. Sephadex II, upon ion exchange chromatography, yielded three isozymes, A, B, and C. These three were characterized with respect to molecular weight, substrate specificities, and effects of hydrogen ion concentration, EDTA, and divalent cation on reaction velocity. The molecular weights are 8.0 × 104, 8.2 × 104, and 9.0 × 104 respectively. Isozymes A and B are neutral naphthylamidases and preferentially catalyze the hydrolysis of alanine-β-naphthylamide (βNA), whereas isozyme C is a basic naphthylamidase and preferentially catalyzes the hydrolysis of lysine and arginine-βNA. The pH optima for the isozymes are 7.6, 7.6, and 6.7, respectively. All of the isozymes are sensitive to the effects of EDTA. Divalent cations activate the enzymes and reverse inhibition caused by EDTA.
Electrophoresis, Cell-Free System, Sarcina, Hydrogen-Ion Concentration, Naphthalenes, Vibration, Chromatography, DEAE-Cellulose, Amidohydrolases, Isoenzymes, Molecular Weight, Acrylates, Bacterial Proteins, Chromatography, Gel, Fluorometry, Amino Acids, Gels, Edetic Acid
Electrophoresis, Cell-Free System, Sarcina, Hydrogen-Ion Concentration, Naphthalenes, Vibration, Chromatography, DEAE-Cellulose, Amidohydrolases, Isoenzymes, Molecular Weight, Acrylates, Bacterial Proteins, Chromatography, Gel, Fluorometry, Amino Acids, Gels, Edetic Acid
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