On the infectivity of the Sindbis virus nucleocapsid
Copyright policy )On the infectivity of the Sindbis virus nucleocapsid
The nucleocapsid fraction derived either from Sindbis virions or from infected cell cultures was studied with respect to its ability to adsorb to susceptible cells as well as to initiate stages in viral replication. Neither preparation was adsorbed to BHK 21 cells under conditions where virion adsorption could be demonstrated. In addition parental RNA from virions was shown to be converted to an RNAase-resistant 16–20 S form, whereas no evidence for the penetration and conversion of RNA present in viral cores was observed. These studies emphasize the role of envelope proteins in facilitating entry of intact virus into susceptible cells.
Carbon Isotopes, Sucrose, Sulfates, Sodium, Kidney, Tritium, Cell Line, Bile Acids and Salts, Nucleoproteins, Phenols, Cricetinae, Centrifugation, Density Gradient, Animals, Chemical Precipitation, RNA, Viral, Adsorption, Sindbis Virus, Amino Acids, Trichloroacetic Acid, Arboviruses
Carbon Isotopes, Sucrose, Sulfates, Sodium, Kidney, Tritium, Cell Line, Bile Acids and Salts, Nucleoproteins, Phenols, Cricetinae, Centrifugation, Density Gradient, Animals, Chemical Precipitation, RNA, Viral, Adsorption, Sindbis Virus, Amino Acids, Trichloroacetic Acid, Arboviruses
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