DNA sequences of RAPD fragments in artiodactyls
Copyright policy )DNA sequences of RAPD fragments in artiodactyls
A bovine RAPD profile, generated by a 10-mer primer, was analysed by sequencing the major fragments. Three of four different fragments showed homologies to previously characterized mammalian sequences. One was 61–66% identical to LINE sequences and another was 78.5% identical to a human chromosome 2 sequence tagged site. The third fragment was 93.1% identical to the human type 2 inositol 1,4,5-trisphosphate receptor gene. This fragment had counterparts in white-tailed deer and reindeer; fragments of slightly different size in these species showed high sequence similarity and the size differences were due to varying numbers of dinucleotide microsatellite repeats inside the fragment. Key words : RAPD, artiodactyls, sequence similarity, microsatellites, type 2 inositol 1,4,5-trisphosphate receptor.
- University of Helsinki Finland
Male, Base Sequence, Deer, Molecular Sequence Data, Receptors, Cytoplasmic and Nuclear, DNA, Pedigree, Random Amplified Polymorphic DNA Technique, Chromosomes, Human, Pair 2, Sequence Homology, Nucleic Acid, Animals, Humans, Inositol 1,4,5-Trisphosphate Receptors, Cattle, Female, Calcium Channels, Artiodactyla, DNA Primers, Reindeer, Sequence Tagged Sites
Male, Base Sequence, Deer, Molecular Sequence Data, Receptors, Cytoplasmic and Nuclear, DNA, Pedigree, Random Amplified Polymorphic DNA Technique, Chromosomes, Human, Pair 2, Sequence Homology, Nucleic Acid, Animals, Humans, Inositol 1,4,5-Trisphosphate Receptors, Cattle, Female, Calcium Channels, Artiodactyla, DNA Primers, Reindeer, Sequence Tagged Sites
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