In the course of a preliminary chemosystematic study of Asphodelus species, six flavonoids were first isolated and identified from A. ramosus subsp. ramosus var. ramosus. Only flavone structures were found. Two of them are rare C-glycoside structures and were observed only in the samples from one of the two geographic areas. So, flavonoids might be used as markers in the study of the Asphodelus genus organization. Key words: Asphodelus ramosus, Liliaceae, flavonoids, C-glycosylflavones, chemosystematics.