Epicuticular waxes from 34 species in 28 genera in 11 tribes (excluding the Triticeae) in four subfamilies of the Gramineae have been analyzed. Amounts and compositions of hydrocarbons, esters, free alcohols, free acids, and β-diketones were determined. There were no species with higher wax contents or greater amounts of esters than had been found in the Triticeae but species with wax containing 50% of hydrocarbons were found. Members from different subfamilies had distinctive free alcohol compositions. Waxes from almost all of the festucoid species contained free alcohols consisting mainly of either hexacosanol or octacosanol but free alcohols from the panicoid species ranged from hexacosanol to dotriacontanol with the latter sometimes predominating. Compositions of alcohols from four eragrostoid genera were like the panicoid type and those from the other two eragrostoid genera were like the festucoid type. Alcohols from one arundinoid genus resembled the panicoid type and from the other resembled the festucoid type.