Multispectral images (MSI) contain light information in different wavelengths of objects, which convey spectral-spatial information and help improve the performance of various image processing tasks. Numerous techniques have been created to extend the application of total variation regularization in restoring multispectral images, for example, based on channel coupling and adaptive total variation regularization. The primary contribution of this paper is to propose and develop a new multispectral total variation regularization in a generalized opponent transformation domain instead of the original multispectral image domain. Here opponent transformations for multispectral images are generalized from a well-known opponent transformation for color images. We will explore the properties of generalized opponent transformation total variation (GOTTV) regularization and the corresponding optimization formula for multispectral image restoration. To evaluate the effectiveness of the new GOTTV method, we provide numerical examples that showcase its superior performance compared to existing multispectral image total variation methods, using criteria such as MPSNR and MSSIM.