Gemella bacteraemia characterised by 16S ribosomal RNA gene sequencing
Copyright policy )Gemella bacteraemia characterised by 16S ribosomal RNA gene sequencing
To define epidemiology, clinical disease, and outcome of gemella bacteraemia by 16S rRNA gene sequencing. To examine the usefulness of the Vitek, API, and ATB systems in identifying two gemella species.All alpha haemolytic streptococci other than Streptococcus pneumoniae isolated from blood cultures during a six year period were identified by conventional biochemical methods, the Vitek system, and the API system. 16S rRNA gene sequencing was performed on all isolates identified by both kits as gemella with >or= 95% confidence or by either kit as any bacterial species with 70% confidence.Gemella bacteraemia is uncommon. 16S rRNA gene sequencing is the method of choice for identification of gemella and gemella-like isolates.
- University of Hong Kong China (People's Republic of)
- Queen Mary Hospital China (People's Republic of)
- Hospital Authority China (People's Republic of)
- University of Hong Kong (香港大學) China (People's Republic of)
Adult, Male, 610, Bacteremia, Polymerase Chain Reaction, Sensitivity and Specificity, Staphylococcaceae, Immunocompromised Host, RNA, Ribosomal, 16S, Humans, Aged, Bone Marrow Transplantation, Ribosomal, Cross Infection - diagnosis, Cross Infection, Staphylococcaceae - genetics, Bacteremia - diagnosis, RNA, Ribosomal, 16S - analysis, Endocarditis, Bacterial, Staphylococcal Infections, Staphylococcal Infections - diagnosis, 16S - analysis, RNA, Female, Sequence Alignment
Adult, Male, 610, Bacteremia, Polymerase Chain Reaction, Sensitivity and Specificity, Staphylococcaceae, Immunocompromised Host, RNA, Ribosomal, 16S, Humans, Aged, Bone Marrow Transplantation, Ribosomal, Cross Infection - diagnosis, Cross Infection, Staphylococcaceae - genetics, Bacteremia - diagnosis, RNA, Ribosomal, 16S - analysis, Endocarditis, Bacterial, Staphylococcal Infections, Staphylococcal Infections - diagnosis, 16S - analysis, RNA, Female, Sequence Alignment
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