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Recombinant oxyntomodulin

descriptionPublicationkeyboard_double_arrow_right Article , Other literature type 01 Mar 2007Publisher:Pleiades Publishing LtdJournal:Russian Journal of Bioorganic Chemistry, volume 33, pages 227-232 (issn: 1068-1620, eissn: 1573-9163,

Authors: Vasily N. Stepanenko; Gurevich Ai; L. A. Chupova; Roman S. Esipov; A. I. Miroshnikov;

doi: 10.1134/s1068162007020045

pmid: 17476985

Recombinant oxyntomodulin

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Abstract

An artificial gene encoding oxyntomodulin was obtained using chemical and enzymatic methods and cloned into Escherichia coli. A recombinant plasmid was constructed containing a hybrid oxyntomodulin gene and Ssp dnaB intein from Synechocystis sp. The expression of the resulting hybrid gene in E. coli, its properties, and the conditions of its autocatalytic cleavage to oxyntomodulin were studied.

Related Organizations

Russian Academy of Sciences
Russian Federation

Keywords

Recombinant Fusion Proteins, Molecular Sequence Data, Synechocystis, Catalysis, Recombinant Proteins, Inteins, Oxyntomodulin, Mutation, Escherichia coli, Amino Acid Sequence, DnaB Helicases, Plasmids

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