An endoplasmic reticulum-specific cyclophilin.
Copyright policy )An endoplasmic reticulum-specific cyclophilin.
Cyclophilin is a ubiquitously expressed cytosolic peptidyl-prolyl cis-trans isomerase that is inhibited by the immunosuppressive drug cyclosporin A. A degenerate oligonucleotide based on a conserved cyclophilin sequence was used to isolate cDNA clones representing a ubiquitously expressed mRNA from mice and humans. This mRNA encodes a novel 20-kDa protein, CPH2, that shares 64% sequence identity with cyclophilin. Bacterially expressed CPH2 binds cyclosporin A and is a cyclosporin A-inhibitable peptidyl-prolyl cis-trans isomerase. Cell fractionation of rat liver followed by Western blot (immunoblot) analysis indicated that CPH2 is not cytosolic but rather is located exclusively in the endoplasmic reticulum. These results suggest that cyclosporin A mediates its effect on cells through more than one cyclophilin and that cyclosporin A-induced misfolding of T-cell membrane proteins normally mediated by CPH2 plays a role in immunosuppression.
- Scripps Health United States
Base Sequence, Molecular Sequence Data, Nucleic Acid Hybridization, Cyclosporins, DNA, Peptidylprolyl Isomerase, Blotting, Northern, Endoplasmic Reticulum, Kinetics, Mice, Liver, Animals, Humans, RNA, Amino Acid Sequence, Cloning, Molecular, Carrier Proteins, Oligonucleotide Probes, Amino Acid Isomerases, Gene Library
Base Sequence, Molecular Sequence Data, Nucleic Acid Hybridization, Cyclosporins, DNA, Peptidylprolyl Isomerase, Blotting, Northern, Endoplasmic Reticulum, Kinetics, Mice, Liver, Animals, Humans, RNA, Amino Acid Sequence, Cloning, Molecular, Carrier Proteins, Oligonucleotide Probes, Amino Acid Isomerases, Gene Library
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