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Cytopathogenicity of a pestivirus correlates with a 27-nucleotide insertion

descriptionPublicationkeyboard_double_arrow_right Article 01 Nov 1996Publisher:American Society for MicrobiologyJournal:Journal of Virology, volume 70, pages 7,851-7,858 (issn: 0022-538X, eissn: 1098-5514,

Authors: Robert Stark; G Meyers; Heinz-Jürgen Thiel; Edward J. Dubovi; Norbert Tautz;

doi: 10.1128/jvi.70.11.7851-7858.1996

pmid: 8892907

pmc: PMC190856

Cytopathogenicity of a pestivirus correlates with a 27-nucleotide insertion

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- Related research
  (6)
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Abstract

Cytopathogenic (cp) bovine viral diarrhea virus (BVDV) strains are generated in cattle persistently infected with noncytopathogenic (noncp) BVDV.cp BVDV strains are considered crucial for the development of fatal mucosal disease. Comparative analysis of cp and noncp BVDV strains isolated from one animal suffering from mucosal disease revealed that the genomes of the cp BVDV strain (CP7) and the corresponding noncp BVDV strain (NCP7) are highly homologous. However, only the genome of CP7 contains an insertion of 27 nucleotides in the NS2 coding region. The inserted sequence represents a duplication of bases 4064 to 4090 of the viral genome, located between the formerly neighboring nucleotides 4353 and 4354. Parts of the viral polyproteins of CP7 and NCP7 were expressed in the T7 vaccinia virus system. These studies revealed that the insertion identified in the CP7 genome is necessary and sufficient for the induction of NS2-3 cleavage. Since the expression of NS3 is strictly correlated to cp BVDV, the insertion identified in the genome of BVDV CP7 represents most likely the relevant mutation leading to the evolvement of CP7 from NCP7.

Related Organizations

Justus Liebig University Giessen
Germany

Keywords

DNA, Complementary, Diarrhea Viruses, Bovine Viral, Base Sequence, Molecular Sequence Data, Serine Endopeptidases, Sequence Analysis, DNA, Viral Nonstructural Proteins, Blotting, Northern, Cell Line, Mutagenesis, Insertional, Structure-Activity Relationship, Cytopathogenic Effect, Viral, Cricetinae, DNA, Viral, Animals, Cattle, Cloning, Molecular, Protein Processing, Post-Translational

6 Research products, page 1 of 1

Bovine viral diarrhea virus cytopathogenic strain CP7, polyprotein gene, E2region to NS4B, partial cds.
2018IsRelatedTo
Bovine viral diarrhea virus noncytopathogenic strain NCP7, polyproteingene, E2 region to NS4B, partial cds.
2018IsRelatedTo
Bovine viral diarrhea virus 1 partial polyprotein
2018IsRelatedTo
Genome polyprotein
2017IsRelatedTo
Bovine viral diarrhea virus 1 partial polyprotein
2018IsRelatedTo
Polyprotein
2017IsRelatedTo

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	citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	70
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