Monoclonal antibodies to Nocardia asteroides and Nocardia brasiliensis antigens
Copyright policy )Monoclonal antibodies to Nocardia asteroides and Nocardia brasiliensis antigens
Nocardia asteroides and Nocardia brasiliensis whole-cell extracts were used as antigens to generate monoclonal antibodies (MAbs). Six stable hybrid cell lines secreting anti-Nocardia spp. MAbs were obtained. These were characterized by enzyme-linked immunosorbent assay, Western blot (immunoblot), and immunofluorescence assay. Although all the MAbs exhibited different degrees of cross-reactivity with N. asteroides and N. brasiliensis antigens as well as with culture-filtrate antigens from Mycobacteria spp., they have the potential for use as reagents in the purification of Nocardia antigens.
- University of Puerto Rico Spain
- UNIVERSITY OF PUERTO RICO
- University of Puerto Rico System United States
- University of Puerto Rico at Río Piedras United States
Antigens, Bacterial, Blotting, Western, Antibodies, Monoclonal, Fluorescent Antibody Technique, Nocardia Infections, Enzyme-Linked Immunosorbent Assay, Cross Reactions, Antibodies, Bacterial, Nocardia, Mycobacterium, Nocardia asteroides, Humans
Antigens, Bacterial, Blotting, Western, Antibodies, Monoclonal, Fluorescent Antibody Technique, Nocardia Infections, Enzyme-Linked Immunosorbent Assay, Cross Reactions, Antibodies, Bacterial, Nocardia, Mycobacterium, Nocardia asteroides, Humans
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