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Simultaneous Detection of Influenza A, Influenza B, and Respiratory Syncytial Viruses and Subtyping of Influenza A H3N2 Virus and H1N1 (2009) Virus by Multiplex Real-Time PCR

descriptionPublicationkeyboard_double_arrow_right Article 01 Apr 2011 English Publisher:American Society for MicrobiologyJournal:Journal of Clinical Microbiology, volume 49, pages 1,653-1,656 (issn: 0095-1137, eissn: 1098-660X,

Authors: Weilin Mao; Jia Tong; Zhenjin Chen; Xuefen Li; Bin Lou; Bo Ye; Genyun Xu; +12 Authors

doi: 10.1128/jcm.02184-10

pmid: 21270233

pmc: PMC3122825

Simultaneous Detection of Influenza A, Influenza B, and Respiratory Syncytial Viruses and Subtyping of Influenza A H3N2 Virus and H1N1 (2009) Virus by Multiplex Real-Time PCR

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Abstract

ABSTRACT A multiplex real-time PCR assay was developed to simultaneously detect and discriminate influenza A virus subtypes, including novel H1N1 (2009) and seasonal H3N2 virus, influenza B virus, and respiratory syncytial virus (RSV) in a single test tube, with detection sensitivity and specificity of 99% and 100%, respectively, for the four pathogens.

Related Organizations

Zhejiang Ocean University
China (People's Republic of)
Jiangsu University
China (People's Republic of)
National Center for Clinical Laboratories
China (People's Republic of)
Wenzhou Medical University
China (People's Republic of)
State Key Laboratory of Diagnosis and Treatment of Infectious Diseases
China (People's Republic of)

Keywords

Clinical Laboratory Techniques, Influenza A Virus, H3N2 Subtype, Respiratory Syncytial Virus Infections, Polymerase Chain Reaction, Sensitivity and Specificity, Respiratory Syncytial Viruses, Influenza B virus, Influenza A Virus, H1N1 Subtype, Virology, Influenza, Human, Humans

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	citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	41
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	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Top 10%
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Top 10%