Expanded Multilocus Sequence Typing for  Burkholderia  Species

descriptionPublicationkeyboard_double_arrow_right Article 01 Aug 2009 English Publisher:American Society for MicrobiologyJournal:Journal of Clinical Microbiology, volume 47, pages 2,607-2,610 (issn: 0095-1137, eissn: 1098-660X,

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Authors: Spilker, T.; Baldwin, A.; Bumford, A.; Dowson, C. G; Mahenthiralingam, Eshwar; LiPuma, J. J.;

doi: 10.1128/jcm.00770-09

pmid: 19494070

pmc: PMC2725695

Expanded Multilocus Sequence Typing for Burkholderia Species

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Abstract

ABSTRACT PCR primers targeting loci in the current Burkholderia cepacia complex multilocus sequence typing scheme were redesigned to (i) more reliably amplify these loci from B . cepacia complex species, (ii) amplify these same loci from additional Burkholderia species, and (iii) enable the use of a single primer set per locus for both amplification and DNA sequencing.

Related Organizations

University of Warwick
United Kingdom
Cardiff University
United Kingdom
University of Michigan–Flint
United States
University of Michigan–Ann Arbor
United States

Keywords

DNA, Bacterial, Burkholderia, Cluster Analysis, Sequence Analysis, DNA, QH426, DNA Fingerprinting, Phylogeny, Bacterial Typing Techniques, DNA Primers

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