Rapid Molecular Strategy for Orbivirus Detection and Characterization
Copyright policy )Rapid Molecular Strategy for Orbivirus Detection and Characterization
ABSTRACT Orbiviruses infect a wide range of hosts, including humans. The ability to detect them has been hampered by their diversity. Here we present a simple consensus reverse transcription (RT)-PCR method targeting the polymerase gene for orbivirus recognition and characterization. Phylogenetic assignment is achieved by automated Web-based sequence analysis of amplification products.
- Colorado State University United States
- Columbia University
- King’s University United States
- Columbia University United States
- Columbia University United States
Internet, Reverse Transcriptase Polymerase Chain Reaction, Molecular Sequence Data, Sequence Analysis, DNA, RNA-Dependent RNA Polymerase, Reoviridae Infections, Viral Proteins, Sequence Homology, Nucleic Acid, Virology, Animals, Cluster Analysis, Humans, RNA, Viral, Orbivirus, Phylogeny
Internet, Reverse Transcriptase Polymerase Chain Reaction, Molecular Sequence Data, Sequence Analysis, DNA, RNA-Dependent RNA Polymerase, Reoviridae Infections, Viral Proteins, Sequence Homology, Nucleic Acid, Virology, Animals, Cluster Analysis, Humans, RNA, Viral, Orbivirus, Phylogeny
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