
A Bacillus subtilis gene for malate dehydrogenase (citH) was found downstream of genes for citrate synthase and isocitrate dehydrogenase. Disruption of citH caused partial auxotrophy for aspartate and a requirement for aspartate during sporulation. In the absence of aspartate, citH mutant cells were blocked at a late stage of spore formation.
Spores, Bacterial, Aspartic Acid, Base Sequence, Sequence Homology, Amino Acid, Recombinant Fusion Proteins, Molecular Sequence Data, Citrate (si)-Synthase, Gene Expression Regulation, Bacterial, Sequence Analysis, DNA, Isocitrate Dehydrogenase, Genes, Bacterial, Malate Dehydrogenase, Mutation, Amino Acid Sequence, Promoter Regions, Genetic, Bacillus subtilis
Spores, Bacterial, Aspartic Acid, Base Sequence, Sequence Homology, Amino Acid, Recombinant Fusion Proteins, Molecular Sequence Data, Citrate (si)-Synthase, Gene Expression Regulation, Bacterial, Sequence Analysis, DNA, Isocitrate Dehydrogenase, Genes, Bacterial, Malate Dehydrogenase, Mutation, Amino Acid Sequence, Promoter Regions, Genetic, Bacillus subtilis
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