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Journal of Bacteriology
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Journal of Bacteriology
Article . 1985 . Peer-reviewed
License: ASM Journals Non-Commercial TDM
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Journal of Bacteriology
Article . 1985
Data sources: Europe PubMed Central
https://dx.doi.org/10.1128/jb....
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Cloning vector system for Corynebacterium glutamicum

descriptionPublicationkeyboard_double_arrow_right Article 01 May 1985 English Publisher:American Society for MicrobiologyJournal:Journal of Bacteriology, volume 162, pages 591-597 (issn: 0021-9193, eissn: 1098-5530, Copyright policy )
Authors: M, Yoshihama; K, Higashiro; E A, Rao; M, Akedo; W G, Shanabruch; M T, Follettie; G C, Walker; +1 Authors

doi: 10.1128/jb.162.2.591-597.1985

pmid: 3921526

pmc: PMC218889

Cloning vector system for Corynebacterium glutamicum

Abstract

A protoplast transformation system has been developed for Corynebacterium glutamicum by using a C. glutamicum-Bacillus subtilis chimeric vector. The chimera was constructed by joining a 3.0-kilobase cryptic C. glutamicum plasmid and the B. subtilis plasmid pBD10. The neomycin resistance gene on the chimera, pHY416, was expressed in C. glutamicum, although the chloramphenicol resistance gene was not. The various parameters in the transformation protocol were analyzed separately and optimized. The resulting transformation system is simple and routinely yields 10(4) transformants per microgram of plasmid DNA.

Keywords

Transformation, Genetic, Gene Expression Regulation, Genetic Vectors, Cloning, Molecular, Corynebacterium, Bacillus subtilis, Plasmids

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
141
Top 10%
Top 1%
Top 10%
bronze