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Applied and Environmental Microbiology
Article
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Applied and Environmental Microbiology
Article . 2000 . Peer-reviewed
License: ASM Journals Non-Commercial TDM
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Applied and Environmental Microbiology
Article . 2000
Data sources: Europe PubMed Central
https://dx.doi.org/10.1128/aem...
Article
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Engineering Desiccation Tolerance in Escherichia coli

descriptionPublicationkeyboard_double_arrow_right Article 01 Apr 2000 English Publisher:American Society for MicrobiologyJournal:Applied and Environmental Microbiology, volume 66, pages 1,680-1,684 (issn: 0099-2240, eissn: 1098-5336, Copyright policy )
Authors: D, Billi; D J, Wright; R F, Helm; T, Prickett; M, Potts; J H, Crowe;

doi: 10.1128/aem.66.4.1680-1684.2000

pmid: 10742260

pmc: PMC92041

Engineering Desiccation Tolerance in Escherichia coli

Abstract

ABSTRACT Recombinant sucrose-6-phosphate synthase (SpsA) was synthesized in Escherichia coli BL21DE3 by using the spsA gene of the cyanobacterium Synechocystis sp. strain PCC 6803. Transformants exhibited a 10,000-fold increase in survival compared to wild-type cells following either freeze-drying, air drying, or desiccation over phosphorus pentoxide. The phase transition temperatures and vibration frequencies (PO stretch) in phospholipids suggested that sucrose maintained membrane fluidity during cell dehydration.

Related Organizations
Keywords

Sucrose, Freeze Drying, Transformation, Genetic, Glucosyltransferases, Escherichia coli, Desiccation, Phosphorus Compounds, Cyanobacteria, Genetic Engineering

  • BIP!
    Impact byBIP!
    selected citations
    These citations are derived from selected sources.
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 78
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    		 Top 10%
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 Top 10%
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    		 Top 10%
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
78
Top 10%
Top 10%
Top 10%
bronze