Immunological detection of Salmonella paratyphi A in raw prawns
Copyright policy )Immunological detection of Salmonella paratyphi A in raw prawns
A slot blot enzyme-linked immunosorbent assay, using monoclonal antibodies specific only for Salmonella paratyphi A, to detect S. paratyphi A contamination in raw prawns has been established. When artificially contaminated prawn samples were tested. S. paratyphi A contamination could be identified correctly within 20 h. No false positives from samples artificially contaminated by other microorganisms were obtained. The sensitivity was such that as few as 1 S. paratyphi A organism per g of raw prawn could be detected. Therefore, the assay constituted a promising test for the rapid and specific detection of S. paratyphi A in prawns.
- Mahidol University Thailand
- Siriraj Hospital Thailand
Antibodies, Monoclonal, Enzyme-Linked Immunosorbent Assay, Antibodies, Bacterial, Sensitivity and Specificity, Species Specificity, Decapoda, Salmonella paratyphi A, Animals, Flagellin, Shellfish
Antibodies, Monoclonal, Enzyme-Linked Immunosorbent Assay, Antibodies, Bacterial, Sensitivity and Specificity, Species Specificity, Decapoda, Salmonella paratyphi A, Animals, Flagellin, Shellfish
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