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Applied and Environmental Microbiology
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Applied and Environmental Microbiology
Article . 1984 . Peer-reviewed
License: ASM Journals Non-Commercial TDM
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Applied and Environmental Microbiology
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Properties of Alginate Lyases from Marine Bacteria

descriptionPublicationkeyboard_double_arrow_right Article 01 Apr 1984 English Publisher:American Society for MicrobiologyJournal:Applied and Environmental Microbiology, volume 47, pages 699-703 (issn: 0099-2240, eissn: 1098-5336, Copyright policy )
Authors: R. Scott Doubet; Ralph S. Quatrano;

doi: 10.1128/aem.47.4.699-703.1984

pmid: 16346509

pmc: PMC239751

Properties of Alginate Lyases from Marine Bacteria

Abstract

Alginate lyases (EC 4.2.2.3) from two marine bacteria were isolated and partially characterized. A cell-bound lyase from isolate A3 had a molecular weight of approximately 100,000 and cleaved mannuronate blocks, apparently in an exo manner. A lyase recovered from the culture medium of isolate W3 was soluble in saturated ammonium sulfate, cleaved guluronate blocks, apparently in an endo manner, and had a molecular weight of 35,000. The thiobarbiturate test and urea-polyacrylamide gel electrophoresis were used to determine substrate specificity and mode of substrate cleavage by the enzymes.

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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
40
Average
Top 10%
Top 10%
bronze