
ABSTRACT We have purified flavin mononucleotide (FMN) from a flavoprotein-overexpressing Escherichia coli strain by cofactor trapping. This approach uses an overexpressed flavoprotein to trap FMN, which is thus removed from the cascade regulating FMN production in E. coli . This, in turn, allows the isolation of highly pure FMN.
Flavoproteins, Flavin Mononucleotide, Escherichia coli Proteins, Coenzymes, Escherichia coli, Gene Expression Regulation, Bacterial, Biotechnology
Flavoproteins, Flavin Mononucleotide, Escherichia coli Proteins, Coenzymes, Escherichia coli, Gene Expression Regulation, Bacterial, Biotechnology
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