Penicillin-Binding Proteins in Leptospira interrogans
Copyright policy )Penicillin-Binding Proteins in Leptospira interrogans
ABSTRACT The Leptospira interrogans ponA and pbpB genes were isolated and characterized. ponA and pbpB encode the penicillin-binding proteins (PBPs) 1 and 3, respectively. There is little sequence variation between the PBP genes from two L. interrogans strains (serovar icterohaemorrhagiae strain Verdun and serovar pomona strain RZ11). The deduced L. interrogans PBP 1 and PBP 3 protein sequences from the two strains shared over 50% similarity to homologous proteins from Escherichia coli . It was demonstrated for strain Verdun that ponA and pbpB are transcribed individually from their own promoter. The ponA and pbpB genes from both strains are separated by 8 to 10 kb and oriented such that their transcription is convergent. The L. interrogans PBP 1 and PBP 3 proteins were synthesized in E. coli and were modified with ampicillin using a digoxigenin-ampicillin conjugate. These data show that both genes encode functional PBPs.
- University of Queensland Australia
- United States Department of Agriculture United States
- National Animal Disease Center United States
- Institut Pasteur France
- University of Queensland Australia
570, Transcription, Genetic, Leptospira interogans, Penicillins, Muramoylpentapeptide Carboxypeptidase, Microbiology, Beta-lactamases, C1, Bacterial Proteins, Multienzyme Complexes, Penicillin-Binding Proteins, Pharmacology & Pharmacy, Amino Acid Sequence, Serotyping, DNA Primers, Sequence Homology, Amino Acid, Reverse Transcriptase Polymerase Chain Reaction, Escherichia coli Proteins, Adenosine Monophosphate, 270301 Bacteriology, Molecular Weight, Hexosyltransferases, Peptidyl Transferases, Penicillin-binding proteins, Peptidoglycan Glycosyltransferase, 730101 Infectious diseases, Leptospira interrogans, Carrier Proteins
570, Transcription, Genetic, Leptospira interogans, Penicillins, Muramoylpentapeptide Carboxypeptidase, Microbiology, Beta-lactamases, C1, Bacterial Proteins, Multienzyme Complexes, Penicillin-Binding Proteins, Pharmacology & Pharmacy, Amino Acid Sequence, Serotyping, DNA Primers, Sequence Homology, Amino Acid, Reverse Transcriptase Polymerase Chain Reaction, Escherichia coli Proteins, Adenosine Monophosphate, 270301 Bacteriology, Molecular Weight, Hexosyltransferases, Peptidyl Transferases, Penicillin-binding proteins, Peptidoglycan Glycosyltransferase, 730101 Infectious diseases, Leptospira interrogans, Carrier Proteins
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