Characterization of beta-lactamases in situ on polyacrylamide gels

descriptionPublicationkeyboard_double_arrow_right Article 01 Dec 1986 English Publisher:American Society for MicrobiologyJournal:Antimicrobial Agents and Chemotherapy, volume 30, pages 951-952 (issn: 0066-4804, eissn: 1098-6596,

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Authors: C C, Sanders; W E, Sanders; E S, Moland;

doi: 10.1128/aac.30.6.951

pmid: 3492960

pmc: PMC180628

Characterization of beta-lactamases in situ on polyacrylamide gels

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Abstract

An inhibitor-based characterization system which allowed the identification of beta-lactamases after isoelectric focusing on polyacrylamide gels was developed. This system, using potassium clavulanate and oxacillin, distinguished type I chromosomally mediated enzymes from other beta-lactamases of gram-negative bacteria.

Keywords

Clavulanic Acids, Gram-Negative Bacteria, Isoelectric Focusing, Cloxacillin, beta-Lactamases

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