The Ccr4-Not complex monitors the translating ribosome for codon optimality
Copyright policy )The Ccr4-Not complex monitors the translating ribosome for codon optimality
Coupling translation and mRNA decay Gene expression requires messenger RNAs (mRNAs)—DNA-derived blueprints of genes—to be translated by protein-producing ribosomes. The levels of mRNAs are tightly regulated, in part by controlling their half-lives. In eukaryotic cells, mRNA half-life is largely linked to translational efficiency, but the mechanism underlying this link has remained elusive. Buschauer et al. used cryo–electron microscopy and RNA sequencing to show how a key regulator of mRNA degradation, the Ccr4-Not complex, monitors the ribosome during mRNA translation. They found that the Not5 subunit directly binds to a ribosomal site exposed specifically during inefficient decoding, thereby triggering mRNA degradation. Analysis of mutants revealed the importance of this sensing mechanism for mRNA homeostasis. Science , this issue p. eaay6912
- Tohoku University Japan
- Ludwig-Maximilians-Universität München Germany
- Cleveland University United States
- University of Munich Germany
- Case Western Reserve University United States
Protein Conformation, alpha-Helical, Saccharomyces cerevisiae Proteins, RNA Stability, Ubiquitin-Protein Ligases, Cryoelectron Microscopy, Peptide Chain Elongation, Translational, Ubiquitination, RNA-Binding Proteins, Eukaryotic Translation Initiation Factor 5A, Saccharomyces cerevisiae, Repressor Proteins, Ribonucleases, Peptide Initiation Factors, Multiprotein Complexes, RNA, Messenger, Codon, Ribosomes, Transcription Factors
Protein Conformation, alpha-Helical, Saccharomyces cerevisiae Proteins, RNA Stability, Ubiquitin-Protein Ligases, Cryoelectron Microscopy, Peptide Chain Elongation, Translational, Ubiquitination, RNA-Binding Proteins, Eukaryotic Translation Initiation Factor 5A, Saccharomyces cerevisiae, Repressor Proteins, Ribonucleases, Peptide Initiation Factors, Multiprotein Complexes, RNA, Messenger, Codon, Ribosomes, Transcription Factors
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