Antibodies to Histones and Histone-Histone Complexes: Immunochemical Evidence for Secondary Structure in Histone 1

descriptionPublicationkeyboard_double_arrow_right Article 30 Apr 1976 English Publisher:American Association for the Advancement of Science (AAAS)Journal:Science, volume 192, pages 469-471 (issn: 0036-8075, eissn: 1095-9203,

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Authors: N, Mihalakis; O J, Miller; B F, Erlanger;

doi: 10.1126/science.3853

pmid: 3853

Antibodies to Histones and Histone-Histone Complexes: Immunochemical Evidence for Secondary Structure in Histone 1

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Abstract

Highly specific antibodies were raised to histone 1 (H1) and the histone complexes H3 2 -H4 2 and H2A-H2B, isolated by salt extraction. Antibody to H1 could detect irreversible conformational changes in acid- or urea-treated H1. The antibodies showed different reactivities with chromosomes as compared to antibodies in acid-extracted histones and should be useful in studies of native chromatin and chromosome structure.

Related Organizations

King’s University
United States
Columbia University
United States

Keywords

Histones, Antibody Specificity, Urea, Hydrogen-Ion Concentration, Antibodies

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