Active Disruption of an RNA-Protein Interaction by a DExH/D RNA Helicase
Copyright policy )Active Disruption of an RNA-Protein Interaction by a DExH/D RNA Helicase
All aspects of cellular RNA metabolism and the replication of many viruses require DExH/D proteins that manipulate RNA in a manner that requires nucleoside triphosphates. Although DExH/D proteins have been shown to unwind purified RNA duplexes, most RNA molecules in the cellular environment are complexed with proteins. It has therefore been speculated that DExH/D proteins may also affect RNA-protein interactions. We demonstrate that the DExH protein NPH-II from vaccinia virus can displace the protein U1A from RNA in an active adenosine triphosphate–dependent fashion. NPH-II increases the rate of U1A dissociation by more than three orders of magnitude while retaining helicase processivity. This indicates that DExH/D proteins can effectively catalyze protein displacement from RNA and thereby participate in the structural reorganization of ribonucleoprotein assemblies.
- Columbia University United States
- Howard Hughes Medical Institute United States
- King’s University United States
- Kettering University United States
Models, Molecular, Binding Sites, Base Sequence, Protein Conformation, Molecular Sequence Data, RNA-Binding Proteins, Nucleoside-Triphosphatase, Acid Anhydride Hydrolases, Ribonucleoprotein, U1 Small Nuclear, Kinetics, Adenosine Triphosphate, Nucleic Acid Conformation, RNA, 3' Untranslated Regions, RNA Helicases, Protein Binding
Models, Molecular, Binding Sites, Base Sequence, Protein Conformation, Molecular Sequence Data, RNA-Binding Proteins, Nucleoside-Triphosphatase, Acid Anhydride Hydrolases, Ribonucleoprotein, U1 Small Nuclear, Kinetics, Adenosine Triphosphate, Nucleic Acid Conformation, RNA, 3' Untranslated Regions, RNA Helicases, Protein Binding
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).282 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 1% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 1% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 1%
Citations provided by BIP!Popularity provided by BIP!