Centriole Duplication in Lysates of Spisula solidissima Oocytes
Copyright policy )Centriole Duplication in Lysates of Spisula solidissima Oocytes
A cell-free system has been developed that executes centriole duplication. Surf clam ( Spisula solidissima ) oocytes, arrested at late prophase of meiosis I, do not contain centrioles, centrosomes, or asters. Serial section high-voltage electron microscopy (HVEM) of asters and spindles isolated from potassium chloride-activated oocytes indicates that within 4 minutes oocytes assemble a single centriole that is duplicated by 15 minutes when assembly of the first meiotic spindle is complete. A mixture of lysates from unactivated oocytes and potassium chloride-activated oocytes induces centriole formation and duplication. Astral microtubule content in these lysate mixtures increases with time.
- Institute of Protein Research Russian Federation
- Russian Academy of Sciences Russian Federation
- State University of New York United States
- Marine Biological Laboratory United States
- State University of New York at Potsdam United States
Meiosis, Microscopy, Electron, Cell-Free System, Tubulin, Oocytes, Animals, Female, Prophase, Bivalvia, Centrioles
Meiosis, Microscopy, Electron, Cell-Free System, Tubulin, Oocytes, Animals, Female, Prophase, Bivalvia, Centrioles
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