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Microinjection of Mouse Eggs

Name: Microinjection of Mouse Eggs
Creator: Lin, T P
Keywords: C3H/HE, Punctures, In Vitro Techniques, Bioassays, Injections, 3. Good health, Mice, Fetus, Pregnancy, Fertilization

descriptionPublicationkeyboard_double_arrow_right Article 21 Jan 1966 United States Publisher:American Association for the Advancement of Science (AAAS)Journal:Science, volume 151, pages 333-337 (issn: 0036-8075, eissn: 1095-9203,

Authors: Lin, T P;

doi: 10.1126/science.151.3708.333

pmid: 4159338

Microinjection of Mouse Eggs

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Abstract

A technique has been developed for injecting known amounts of liquids into fertilized one-celled mouse eggs by use of a calibrated ocular micrometer superimposed on the terminal regions of the injection pipette. From 128 pronuclear eggs, each injected with 180 or 770 cubic microns of bovine gamma globulin at a concentration of 25 milligrams per milliliter in citrate-Locke's solution and then transferred to the oviducts of pregnant foster mothers, 18 living fetuses developed; from 74 eggs, each injected with 2730 cubic microns, 5 fetuses survived. The living fetuses that developed from the injected eggs were smaller than normal in 6 of 23 surviving experimental fetuses as contrasted to only 1 of 19 control fetuses.

Country

United States

Related Organizations

University of California, San Francisco
United States
University of California System
United States
Jackson Laboratory
United States

Keywords

C3H/HE, Punctures, In Vitro Techniques, Bioassays, Injections, Mice, Fetus, Pregnancy, Fertilization, Embryology:, Morphology:, Animals, Pregnancy, Animal, Transplantation, Homologous, Cattle, Female, gamma-Globulins, Techniques:, Strains: A(CAL-A) (A/J), BALB/C, Ovum

Impact byBIP!

	citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	82
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Top 10%
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Top 1%
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Top 10%