The use of binaural time and intensity cues to localize sound can be investigated by determining the ability of a subject to localize pure tones in a free-field. Specifically, the ability to localize low-frequency tones, which do not produce an intensity difference at the two ears, demonstrates the use of the binaural phase (time) cue whereas the ability to localize high-frequency tones, to which the auditory system cannot phase lock, demonstrates the use of the binaural intensity-difference cue. Because previous studies of the laboratory rat (Rattus norvegicus) disagreed on the highest frequency that could be localized using binaural phase difference, the ability of rats to localize pure tones was reexamined. The results indicated that, contrary to previous studies, laboratory rats are not able to localize low-frequency tones even when they are amplitude modulated. Thus, it appears that laboratory rats are unable to use the binaural time-difference cue to localize sound. Because the previous studies were conducted before the widespread availability of spectrum analyzers, it is possible that their results were due to the presence of high-frequency harmonics in their low-frequency tones. These results have relevance for the anatomical and physiological study of binaural processing in laboratory rats. a)Presently at Disney’s Animal Kingdom, Lake Buena Vista, FL.