It is likely that the role of automated audiometry will expand in both clinical and research settings in the next few years. A novel method for measuring pure tone thresholds using an automated threshold measurement method is reported here. The Automated Audiometry for the NIH Toolbox (AANT) test was developed for use in the NIH Toolbox multi-disciplinary evaluation battery which contains over 20 tests of sensory function and cognition. Development goals included low system cost, high accuracy, test administration time under 10 minutes, and automated calibration and measurement procedures suitable for use by evaluators without specialized training. Here we report the results of a validation study in which pure tone thresholds obtained using the AANT algorithm and hardware are compared to pure tone thresholds measured using the gold standard clinical method, standard audiometric hardware, and experienced examiners. Air-conduction thresholds will be reported for test frequencies of 500, 1000, 2000, 4000, 6000, and 8000 Hz using both methods. Additional measures for each subject include otoscopy, screening tympanometry, and the Hearing Handicap Inventory for Adults or Elderly. Data will be reported for 100 subjects between the ages of 4 and 85 years in age ranges.