The present investigations were designed to establish the features of vowel spectra that mediate formant frequency discrimination. Thresholds for detecting frequency shifts in the first and second formants of two steady-state vowels were initially measured for conditions in which the amplitudes of all harmonics varied in accordance with a model of cascade formant synthesis. In this model, changes in formant frequency produce level variations in components adjacent to the altered formant as well as in harmonics spectrally remote from the shifted resonant frequency. Discrimination thresholds determined with the cascade synthesis procedure were then compared to difference limens (DLs) obtained when the number of harmonics exhibiting level changes was limited to the frequency region surrounding the altered formant. Results indicated that amplitude variations could be restricted to one to three components near the shifted formant before significant increases in formant frequency DLs were observed. In a second experiment, harmonics remote from the shifted formant were removed from the stimuli. In most cases, thresholds for these reduced-harmonic complexes were not significantly different from those obtained with full-spectrum vowels. Preliminary evaluation of an excitation-pattern model of formant frequency discrimination indicated that such a model can provide good accounts of the thresholds obtained in the present experiments once the salient regions of the vowel spectra have been identified. Implications of these findings for understanding the mechanism mediating vowel perception are discussed.