In this investigation, acoustic radiation force was used as a stimulus to determine the threshold for tactile perception in the human finger and upper forearm as a function of frequency and pulse duration. Initially, a small (1.8-cm2) acoustically reflecting disk was affixed to the anatomical exposure site to maximize the delivered radiation force. Exposures were performed using a 2.2-MHz unfocused source modulated to produce square waves at 50, 100, 200, 500, and 1000 Hz. For the finger, maximum tactile sensitivity occurred at 200 Hz with a threshold radiation force of ∼0.4 mN. For single pulses of 1 to 100 ms at 2.2 MHz, the threshold forces were an order of magnitude greater than for continuous exposure modulated at 200 Hz. Thresholds for pulse durations of 0.1 ms were somewhat greater than for pulses longer than 1 ms. Subsequently, thresholds of tactile perception were determined for direct exposure of the upper forearm (avoiding bone) to single pulses of 2.2-MHz ultrasound. Comparison of perception thresholds with and without a reflecting material over the tissue were consistent with the hypothesis that the tactile sensation experienced when tissue is exposed to ultrasound is its response to the radiation force associated with the transfer of momentum from the sound field to the tissue medium.