Several types of measurements were made to determine the acoustic characteristics that distinguish between voiced and voiceless fricatives in various phonetic environments. The selection of measurements was based on a theoretical analysis that indicated the acoustic and aerodynamic attributes at the boundaries between fricatives and vowels. As expected, glottal vibration extended over a longer time in the obstruent interval for voiced fricatives than for voiceless fricatives, and there were more extensive transitions of the first formant adjacent to voiced fricatives than for the voiceless cognates. When two fricatives with different voicing were adjacent, there were substantial modifications of these acoustic attributes, particularly for the syllable-final fricative. In some cases, these modifications leads to complete assimilation of the voicing feature. Several perceptual studies with synthetic vowel-consonant-vowel stimuli and with edited natural stimuli examined the role of consonant duration, extent and location of glottal vibration, and extent of formant transitions on the identification of the voicing characteristics of fricatives. The perceptual results were in general consistent with the acoustic observations and with expectations based on the theoretical model. The results suggest that listeners base their voicing judgments of intervocalic fricatives on an assessment of the time interval in the fricative during which there is no glottal vibration. This time interval must exceed about 60 ms if the fricative is to be judged as voiceless, except that a small correction to this threshold is applied depending on the extent to which the first-formant transitions are truncated at the consonant boundaries.