Formant frequencies in an old Estonian folk song performed by two female voices were estimated for two back vowels /a/ and /u/, and for two front vowels /e/ and /i/. Comparison of these estimates with formant frequencies in spoken Estonian vowels indicates a trend of the vowels to be clustered into two sets of front and back ones in the F1/F2 plane. Similar clustering has previously been shown to occur in opera and choir singing, especially with increasing fundamental frequency. The clustering in the present song, however, may also be due to a tendency for a mid vowel to be realized as a higher-beginning diphthong, which is characteristic of the North-Estonian coastal dialect area where the singers come from. No evidence of a ‘‘singer’s formant’’ was found.