Spontaneous otoacoustic emissions (SOAEs) were measured longitudinally for durations up to 19.5 years. Initial ages of the subjects ranged from 6 to 41 years. The most compelling finding was a decrease in frequency of all emissions in all subjects, which was approximately linear in %/year and averaged 0.25%/year. SOAE levels also tended to decrease with age, a trend that was significant, but not consistent across emissions, either within or across subjects. Levels of individual SOAEs might decrease, increase, or remain relatively constant with age. Several types of frequency/level instabilities were noted in which some SOAEs within an ear interacted such that their levels were negatively correlated. These instabilities often persisted for many years. SOAEs were also measured in two females over the course of their pregnancies. No changes in SOAE levels or frequencies were seen, that were larger than have been reported in females over a menstrual cycle, suggesting that levels of female gonadal hormones do not have a significant direct effect on SOAE frequencies or levels.