Passive sound source localization with sensor arrays is based on the estimation of the time difference of arrival (TDOA), and precise TDOA is required to achieve accurate position estimation. For a majority of practical localization systems (based on TDOA estimation with four sensors in two dimensions), only three time delays are computed to determine the location of interest. This paper presents an approach to determine the position of a manatee by using four hydrophones and all the combinations of the TDOAs available. With four hydrophones, six TDOAs are computed and then combined three by three to get 20 possible points for each position to estimate. Experimental results using the Hilbert envelope peak technique to estimate the TDOAs and the least square method to estimate the position are presented. For the tests conducted it is shown that for a manatee call having a high signal-to-noise ratio, the individual position estimated for each of the 20 combinations of TDOAs lies on a straight line, providing a good estimation of the direction of arrival approximately 85% of the time. However, a good estimation of the position is obtained for a manatee near the hydrophone array approximately 55% of the time.