Digital background subtraction for fluorescence imaging

descriptionPublicationkeyboard_double_arrow_right Article 01 Sep 1986 English Publisher:WileyJournal:Medical Physics, volume 13, pages 717-721 (issn: 0094-2405, eissn: 2473-4209,

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Authors: A E, Profio; O J, Balchum; F, Carstens;

doi: 10.1118/1.595836

pmid: 3785001

Digital background subtraction for fluorescence imaging

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Abstract

A method of enhancing contrast in fluorescence imaging has been devised, based on real‐time digital subtraction of a background video image from a signal‐plus‐background video image. Color filters are used to differentiate signal from background. The technique has been applied to detection of small tumors labeled with the tumor specific fluorescent drug hematoporphyrin derivative.

Related Organizations

University of California, Santa Barbara
United States
University of Southern California
United States
University of California System
United States

Keywords

Spectrometry, Fluorescence, Neoplasms, Biophysics, Humans, Signal Processing, Computer-Assisted, Biophysical Phenomena

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