Authors considered the possibility of designing a non-invasive biomedical Raman spectrophotometer for the near infrared range using an ellipsoidal reflector as a collecting and focusing optical element to evaluation the biological media components, particularly glucose level. The technical solution for creating the spectrophotometer and its prototype are presented in this paper. The research of the experimental setup was observed faster and more sensitive photodetector reaction to the dynamic blood glucose changes of concentration. Practical testing was carried out by the Raman shift signal measuring on healthy volunteers at different time intervals with food intake associated. The experimental setup principles can be used to design a portable non-invasive glucometer or to quantitatively analyses the chemical composition of biological objects by changing Raman filters.