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The transition dipole moment representation and spectral phasors

descriptionPublicationkeyboard_double_arrow_right Article , Other literature type 05 Mar 2021Publisher:SPIEJournal:Multiphoton Microscopy in the Biomedical Sciences XXI

Authors: Jessica Kline; Marcos Dantus;

doi: 10.1117/12.2582780

The transition dipole moment representation and spectral phasors

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Abstract

Spectral phasors are currently based on fluorescence spectra in the wavelength domain. However, this is a problem when using spectral phasors to quantify emitters since wavelength represented spectra are not proportional to emitter population. Here we show the effect of unmixing using spectra in their transition dipole moment representation for a theoretical two component system and on a real seven component system.

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Michigan State University
United States

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Fields of Science (4) View all

engineering and technology

nano-technology

Fields of Science

engineering and technology

nano-technology

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