ABSTRACTObjectiveTo compare the results of the measurement of femoral torsion using the axial measurement method on three‐dimensional (3D) volumetric reconstructions of computed tomography images AMM(CT), the biplanar measurement method on 3D volumetric reconstructions of computed tomography images BMM(CT) and a reference standard using the axial measurement method on stereolithographic bone models AMM (SBM).Study designEx vivo study.Sample populationsThree‐dimensional volumetric reconstructions of computed tomography images and stereolithographic bone models from 23 femurs of 14 dogs with hind limb lameness presented for orthopedic evaluation.MethodsThree‐dimensional volumetric reconstructions of computed tomography images and stereolithographic bone models of each femur were created from computed tomography data. Femoral torsion was measured using the AMM (CT) and the BMM (CT) and compared with a reference standard, the AMM (SBM).ResultsNo differences were noted among the measurement methods (P = .0863). Median measurement of femoral torsion using the AMM (CT) was 34.2°, the BMM (CT) was 36.7°, and the AMM (SBM) was 32.3°.ConclusionNo differences existed among the AMM (CT), the BMM (CT), and the AMM (SBM).Clinical significanceBoth AMM (CT) and BMM (CT) can be used to measure femoral torsion in dogs with orthopedic disease.